RU486 Suppresses Progesterone-induced Acrosome Reaction in Boar Spermatozoa
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چکیده
منابع مشابه
Role of Calcium and Calcium Channels in Progesterone Induced Acrosome Reaction in Caprine Spermatozoa
There are several physiological and pharmacological evidences indicating that opening of voltage dependent Ca channels play a critical role in induction of acrosome reaction in mammalian sperm. We determined the intracellular free Ca concentration in ejaculated goat sperm using a fluorescent, Ca-specific probe, Fura2/AM, after the suspension of sperm in KRB medium, capable of sustaining capacit...
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The mammalian acrosome reaction (AR) is the fusion, at multiple points, of the outer acrosomal membrane with the overlying plasma membrane. This fusion causes the release of acrosomal contents and the exposure of the inner acrosomal membrane. The time taken for this process to occur is not accurately known. Estimates for AR in mouse spermatozoa vary from ;2 minutes after stimulation with solubi...
متن کاملCharacterization of the endocannabinoid system in boar spermatozoa and implications for sperm capacitation and acrosome reaction.
Anandamide (AEA) is the endogenous ligand of cannabinoid (CB) receptors, and as such it plays several central and peripheral activities. Regulation of female fertility by AEA has attracted growing interest, yet a role for this endocannabinoid in controlling sperm function and male fertility in mammals has been scarcely investigated. In this study we report unprecedented evidence that boar sperm...
متن کاملEffects of gamma-amino butyric acid, progesterone and oestradiol on human spermatozoa acrosome reaction
The acrosome reaction (AR) is influenced by the action of several molecules such as GABA, P 4 , and E 2 . Objective: to determine the interaction between GABA / E 2 , and GABA / P 4 in the AR. Methods: Washed human spermatozoa capacitated for 2 h were incubated in the following conditions: BWW medium alone (control) or BWW plus: P 4, E 2, GABA, GABA+ P 4 or GABA+ E 2. The % of AR was determined...
متن کاملEffect of acrosome reaction progress in frozen-thawed boar spermatozoa on the efficiency of in vitro oocyte fertilization
A good functional status of cryopreserved boar spermatozoa is very important for successful fertilization of porcine oocytes and in vitro embryo production. The purpose of the study was to evaluate the changes in functional status of boar spermatozoa separated from frozen-thawed semen and capacitated in vitro by caffeine. The effect of acrosome reaction development in spermatozoa on the efficie...
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ژورنال
عنوان ژورنال: BMB Reports
سال: 2002
ISSN: 1976-6696
DOI: 10.5483/bmbrep.2002.35.6.604